#AusEvol2024 - Depth-based correction of gene duplications and losses in genome assemblies

The Australasian Evolution Society conference has always been one of my favourites, due to its laid back culture of inclusivity and kindness. (And low cost!) It therefore feels quite fitting that my last conference as an Aussie academic was AES2024.

This talk was a bit of an update from my AES2021 presentation. This showcased some of the latest additions to DepthKopy, including depth-based copy number correction of genome features, such as rDNA genes, repeat families, or multicopy genes. This includes a feature that classifies multicopy “Duplicated” genes identified by BUSCO as true (biological) or false (artefactual) duplicates. TL/DR version: analysis of draft genome assemblies for 45 species of fish across five different depths/qualities indicates that DepthKopy can correct the copy number and total length of multicopy features to within 10% of the true number. (The lower-quality raw assemblies ranged from a 30% under-estimate to a 60% over-estimate.)

This will be of most importance when low quality draft genomes are included in a comparative genomics analysis. However, even the best genome assemblies appear to have some “collapsed” or duplicated loci where the copy number in the assembly does not accurately reflect the copy number in the genome. DepthKopy is useful for exploring the magnitude of such disparities, and can help to identify and correct specific disrepancies in genes or features of interest.

So long, Ocean Genomes... and thanks for all the fish!

After a successful couple of years, today was my last day at UWA. I am proud of the team that I helped to build at the Minderoo Oceanomics Centre at UWA, and the things we have accomplished together. The UWA Oceans Institute has been a fantastic place to work, and I look forward to completing some exciting ongoing collaborations in my capacity as adjunct. It's been exciting to see Ocean Genomes grow from a concept with a largely empty lab to a fully-fledged genome factory capable of generating multiple high-quality genomes a week. The associated publications should hopefully be following soon, and I look forward to continued collaboration with the team as an Oceans Institute adjunct.

Developments in DNA sequencing technology over the past few years have been immense, but the most impressive part for me has been witnessing the laboratory technical team optimising the sample preparations for sequencing. Everything gets so much harder when you move from human samples (the focus of most methods development and testing) into non-model organisms, and I am convinced that the quality of genomes we’ve been producing is in large part due to the quality of the DNA going into the sequencers.

I am now looking for my next challenge and am officially Open For Work. We’ll be moving back to Dublin at the end of January. If you are based in Ireland and need an experienced interdisciplinary problem solver with broad expertise across bioinformatics and biomolecular science, please get in touch! Academic and non-academic opportunities are welcome.